Journal: American Journal of Translational Research
Article Title: Long non-coding RNA HULC regulates TLR4 expression by acting as ceRNA to attract miR-663b in skin fibroblasts of pediatric burns
doi:
Figure Lengend Snippet: HULC promotes TLR4 expression by regulating miR-633b. A: The relative concentration of TLR4 in normal skin tissue and burn wound tissue on the 5th day (n=46) after thermal injury; B: Expression of TLR4 in thermally-injured HSF cells; C: Effect of silencing HULC on the activity of TLR4 in thermally-injured HSF cells. D: Overexpression of miR-663b affected the activation of TLR4 in thermally-injured HSF cells; E: Positive correlation between HULC and TLR4 expression in thermally-injured HSF cells; F: Negative correlation between TLR4 and miR-663b in thermally-injured HSF cells; G: Targeted binding site of miR-663b on TLR4; H: Dual-luciferase reporter assay results; I: RIP results. HSF: human skin fibroblasts. Compared with the normal group, *P<0.05. Compared with the Blank group, #P<0.05. Compared with the si-NC group, ^P<0.05. Compared with the miR-NC group, %P<0.05. Compared with the co-transfected miR-NC & TLR4-wt groups, @P<0.05. Compared with the Anti-IgG group, &P<0.05.
Article Snippet: Cell culture Human skin fibroblasts (HSF) were purchased from ATCC in the United States.
Techniques: Expressing, Concentration Assay, Activity Assay, Over Expression, Activation Assay, Binding Assay, Luciferase, Reporter Assay, Transfection